PRACTICAL WORK

Staining of rhizobium in root nodules of legumes

 AIM

     To study the bacteria in root nodules of a leguminous plant.

MATERIAL REQUIRES

   A leguminous plant (mimosa plant ) with nodulated root ,slide, needle, cover slip,microscope ,methylene blue.

PROCEDURE

      The roots nodules taken from mimosa plant are washed well cut & squeezed  the root nodules  using a needle . A white colour liquid was appeared . Remove the solid particles & place the liquid in a glass slide . Added a drop of methylene blue & put a coverslip over it . Examine under it a high power compound microscope .

OBSERVATION
                     The cells of root nodules contain rod shaped bacteria. Each group is bounded by a membrane with in a cell. root nodules are produced by the presence of bacteria are also called Rhizobium leguminosarum.

  submitted by

                                                                 KARTHIKA RAJAN P R