practical work

PRACTICAL WORK

TOPIC : STUDY OF BACTERIA-LACTOBACILLUS

INTRODUCTION

Microorganisms are essential for the structure of biosphere. Many microorganisms have a closed association with human. Microbes are both good and harm. The symbiotic fixing bacteria which are in the root nodules of  Leguminosae  plants enhance the soil fertility. Microbes are also used in the production of food improves and flavoring agent. Microbes are used as experimental organisms for experimental genetic engineering.

AIM  

To study the shape and size of the bacteria lactobacillus and bacteria from root nodules.

MATERIALS REQUIRED

Clean, microscope with high power or immersion objective lens, 1% of methylene blue, spirit lamp, 100 ml beaker containing distilled water, curd.

PROCEDURE

Place a drop of curd on a clean slide spread this drop to form a thin film with the help of another slide held at an angle of 40 degree touching the curd and moreover the first slide. Allow the slide to dry in air, place the slide film by up side  quickly over a spirit lamp and fix the bacteria. Flood the slide with methylene blue to cover the smear complete for five minute. Dip in the beaker contain distilled water for wash gently excessive stain has been remove. Wave the slide vigorously through the air so that the film dries quickly. Observe under the high power microscope. Observing under oil immersion at between the slide condenser as well as between the cover glass and objective oil immersion lens. Watch from the slide bring the oil emerging objective lens. In close contact with the oil on the cover glass and bring the focus.

CAUTION

Too much heat will destroy the normal shape and structure of  bacteria . When  placed again back of your hand the slide should be warm.

RESULT

Individual bacteria can been seen as tiny rods in various shades of blue.

Submitted By ,

Sharika Mohan I

Roll no ;11

Natural science