PRACTICAL WORK
PREPARATION OF TEMPORARY
STAINED SLIDE OF
HUMAN BLOOD
INTRODUCTION
Blood is
a specialized fluid
connective tissue which
acts as the
main transporting system of
the body. Blood are
classified as liquid
and cellular components.
Plasma is the
liquid component and
constitutes about 55% of
the total blood
volume and constitutes
of 91.5% of
water and 8.5%
solutes cellular components
include erythrocytes(RBC),
leucocytes (WBC) and platelets. Blood cells
are formed in
the bone marrow
by process called
heamopoiesis and in
the lymphoid tissue
by lymphopoiesis.
Red blood
corpuscles have a
biconcave disc shape
structure. Haemoglobin give red
colour to the
red blood corpuscles
and hence the
blood is red in colour. The
erythrocytes are non-nucleated and
are more in
number. The average
life span of
erythrocyte is about
128. The primary
function of erythrocytes
is transportation of
respiratory gases regulation of pH of
blood.
AIM
To prepare
a temporary stained
slide of human
blood.
MATERIALS
REQUIRED
Human blood, 70%
alcohol, Leishman’s Wright’s stain,
olive oil, distilled
water, glass slides,
cover slips, needles, spirit lamp, cotton, compound microscope etc.
PROCEDURE
Apply alcohol
to the tip of one of your
finger. Sterilize the
needle immediately before
use by placing
the pointed end
of the needle
on the flame
for a second and
dipping it in
alcohol. Puncture the skin
on the tip
of the finger, squeeze the
finger from the
sides of puncture. Put
one or two
drops of blood
on one end
of a clean
glass slide. Wipe off
the finger with
alcoholic cotton. Place another
clean glass slide
with one end
at a 300 to 400 angle
on the first
slide and touch
the drop of
blood. This would spread
the blood along
the narrow end
of the slide
forming a thin
uniform layer over
the slide. Now push
the upper side
rapidly towards the
opposite end of
the first slide all along touching
the first side. Allow
the blood film
in the air
and adhere to
the slide.
RESULT
The
stained human blood
smear showed numerous
non- nucleated erythrocytes.
ASWATHY M.S.
ROLL NO:6
NATURAL SCIENCE
ASWATHY M.S.
ROLL NO:6
NATURAL SCIENCE
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