Saturday, 14 January 2017
Wednesday, 11 January 2017
Friday, 6 January 2017
PRACTICAL WORK
STUDY OF BACTERIA-LACTOBACILLUS
INTRODUCTION
MICRO-ORGANISMS ARE ESSENTIAL FOR THE STUCTURE OF BIOSPHERE.MANY MICRO-ORGANISMS HAVE A CLOSED ASSOCIATION WITH HUMAN.MICROBES ARE BOTH GOOD AND HARM.THE SYMBIOTIC NITROGEN FIXING BACTERIA WHICH ARE IN HE ROOT NODULES OF LEGUMINOSAE PLANTS ENHANCE THE SOIL FERTILITY.MICROBES ARE ALSO USED IN PRODUCTION OF FOOD IMPROVES AND FLAVORING AGENTS.MICROBES ARE USED AS EXPERIMENTAL ORGANISMS FOR EXPERIMENTAL GENETIC ENGINEERING.
AIM
TO STUDY THE SHAPE AND SIZE OF THE BACTERIA LACTOBACILLUS AND BACTERIA FROM ROOT NODULES.
MATERIALS REQUIRED
CLEAN SLIDE,MICROSCOPE WITH HIGH POWER OR IMMERSIAL OBJECTIVE LENS,1% OF METHYLENE BLUE,SPIRIT LAMP,100ML BEAKER CONTAINING DISTILLED WATER,CURD.
PROCEDURE
PLACE A DROP OF CURD ON A CLEAN SLIDE, SPREAD THIS DROP TO FORMA THIN FILM WITH THE HELP OF ANOTHER SLIDE HELD AT AN ANGLE OF 40 DEGREE TOUCHING THE CURD AND MOREOVER THE FIRST SLIDE.ALLOW THE SLIDE TO DRY IN AIR , PLACE THE SLIDE FILM BY UP SIDE QUICKLY OVER A SPIRIT LAMP AND FIX THE BACTERIA.FLOOD THE SLIDE WITH METHYLENE BLUE TO COVER THE SMEAR COMPLETE FOR 5 MINUTES.DIP IN THE BEAKER CONTAIN DISTILLED WATER FOR WASH GENTLY EXCESSIVE STAIN HAS BEEN REMOVE. WAVE THE SLIDE VIGOUROSLY THROUGH THE AIR SO THAT FILM DRIES QUICKLY.OBSERVING UNDER OIL IMMERSION AT BETWEEN THE SLIDE CONDENSER AS WELL AS BETWEEN THE COVER GLASS AND OBJECTIVE OIL IMMERSION LENS.WATCH FROM THE SLIDE BRING THE OIL EMERGING OBJECTIVE LENS.IN CLOSE CONTACT WITH THE OIL ON THE COVER GLASS AND BRING THE FOCUS.
CAUTION
TOO MUCH HEAT WILL DESTROY THE NORMAL SHAPE AND STRUCTURE OF BACTERIA.WHEN PLACED AGAIN BACK OF YOUR HAND THE SLIDE SHOULD BE WARM.
RESULT
INDIVIDUAL BACTERIA CAN BEEN SEEN AS TINY RODS IN VARIOUS SHADES OF BLUE.
SUBMITTED BY,
SHARIKA
ROLL NO:11
NATURAL SCIENCEMOHAN I
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